Luciferin analog displaying luminescence peak at 677 nm (near-infrared window) with standard firefly luciferase reporter system enabling deeper and more sensitive tissue imaging due to the reduced absorption and scattering of light compared to photonic emissions in the visible wavelengths. The analog also provides stroger emissions for for in vitro assays compared to use of D-Luciferin. Properties Chemical Name: (4S)-2-[(1E,3E)-4-[4-(Dimethylamino)phenyl]-1,3-butadien-1-yl]-4,5-dihydro-4-thiazolecarboxylic acid hydrochloride Molecular Weight: 302.39 Purity: 96% by HPLC Soluble at 5 mg/mL in water and 10 mg/ml in DMSO. A gentle warming in water bath at 45-60°C is recommended for solubilizing in water Reference publication: Kuchimaru T, et al. Nat Commun. 2016 Jun 14;7:11856. doi: 10.1038/ncomms11856. PubMed PMID: 27297211; PubMed Central PMCID: PMC4911627 Download AkaLumine HCL certificate of analysis

Publications

18 articles

• Nanoparticle-based Cell Trackers for Biomedical Applications. 2020, Theranostics, Ni JS, Li Y, Yue W, Liu B, Li K. abstract  article
• Development of a Staphylococcus aureus reporter strain with click beetle red luciferase for enhanced in vivo imaging of experimental bacteremia and mixed infections. 2019, Sci Rep, Miller RJ, Crosby HA, Schilcher K, Wang Y, Ortines RV, Mazhar M, Dikeman DA, Pinsker BL, Brown ID, Joyce DP, Zhang J, Archer NK, Liu H, Alphonse MP, Czupryna J, Anderson WR, Bernthal NM, Fortuno-Miranda L, Bulte JWM, Francis KP, Horswill AR, Miller LS. abstract  article
• Self-Illuminating Agents for Deep-Tissue Optical Imaging. 2019, Front Bioeng Biotechnol, Li Q, Zeng J, Miao Q, Gao M. abstract  article
• In Vivo Tracking of Tissue Engineered Constructs. 2019, Micromachines (Basel), Gil CJ, Tomov ML, Theus AS, Cetnar A, Mahmoudi M, Serpooshan V. abstract  article
• Phrixotrix luciferase and 6'-aminoluciferins reveal a larger luciferin phenolate binding site and provide novel far-red combinations for bioimaging purposes. 2019, Sci Rep, Bevilaqua VR, Matsuhashi T, Oliveira G, Oliveira PSL, Hirano T, Viviani VR. abstract  article
• Bioluminescence and near-infrared fluorescence imaging for detection of metastatic bone tumors. 2020, Lasers Med Sci, Lim W, Kim B, Jo G, Yang DH, Park MH, Hyun H. abstract  article
• Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy: In Vitro and In Vivo Perspectives. 2019, Theranostics, Yan Y, Shi P, Song W, Bi S. abstract  article
• ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. 2019, ACS Chem. Biol., Yeh HW, Xiong Y, Wu T, Chen M, Ji A, Li X, Ai HW. abstract  article
• Identification of Factors Complicating Bioluminescence Imaging. 2019, Biochemistry, Yeh HW, Wu T, Chen M, Ai HW. abstract  article
• Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging. 2018, Nat Commun, Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi C, Kirkland TA, Encell LP, Wood KV, Löwik C, Mezzanotte L, Mezzanotte L. abstract  article

 

  Bioluminescent substrate used for imaging of renilla luciferase with peak emission at 480 nm. Produces a larger initial light intensity compared with Coelenterazine Native

Used for BRET applications with renilla luciferase and is the preferred substrate for Gaussia luciferase which is oxidized after binding of Ca2+ to the aquaporin and emits light with peak emission at 465 nm

Luciferin analog substrate with luminescence peak at 620 nm (orange to red window) when used with the standard firefly luciferase reporter system. This enables deeper and more sensitive tissue imaging due to the reduced absorption and scattering of light compared to photonic emissions of D-Luciferin. The analog also provides stronger emissions for in vitro assays compared to the use of D-Luciferin. Properties Chemical Name: Sodium (4S)-2-(6,7-dihydro-5H-thiazolo[4,5-f]indol-2-yl)-4,5-dihydro-thiazole-4-carboxylate Molecular Weight: 327.36 Purity: 98% by HPLC Reference publication: Kuchimaru T, et al. Nat Commun. 2016 Jun 14;7:11856. doi: 10.1038/ncomms11856. PubMed PMID: 27297211; PubMed Central PMCID: PMC4911627.  

Publications

18 articles

• Nanoparticle-based Cell Trackers for Biomedical Applications. 2020, Theranostics, Ni JS, Li Y, Yue W, Liu B, Li K. abstract  article
• Development of a Staphylococcus aureus reporter strain with click beetle red luciferase for enhanced in vivo imaging of experimental bacteremia and mixed infections. 2019, Sci Rep, Miller RJ, Crosby HA, Schilcher K, Wang Y, Ortines RV, Mazhar M, Dikeman DA, Pinsker BL, Brown ID, Joyce DP, Zhang J, Archer NK, Liu H, Alphonse MP, Czupryna J, Anderson WR, Bernthal NM, Fortuno-Miranda L, Bulte JWM, Francis KP, Horswill AR, Miller LS. abstract  article
• Self-Illuminating Agents for Deep-Tissue Optical Imaging. 2019, Front Bioeng Biotechnol, Li Q, Zeng J, Miao Q, Gao M. abstract  article
• In Vivo Tracking of Tissue Engineered Constructs. 2019, Micromachines (Basel), Gil CJ, Tomov ML, Theus AS, Cetnar A, Mahmoudi M, Serpooshan V. abstract  article
• Phrixotrix luciferase and 6'-aminoluciferins reveal a larger luciferin phenolate binding site and provide novel far-red combinations for bioimaging purposes. 2019, Sci Rep, Bevilaqua VR, Matsuhashi T, Oliveira G, Oliveira PSL, Hirano T, Viviani VR. abstract  article
• Bioluminescence and near-infrared fluorescence imaging for detection of metastatic bone tumors. 2020, Lasers Med Sci, Lim W, Kim B, Jo G, Yang DH, Park MH, Hyun H. abstract  article
• Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy: In Vitro and In Vivo Perspectives. 2019, Theranostics, Yan Y, Shi P, Song W, Bi S. abstract  article
• ATP-Independent Bioluminescent Reporter Variants To Improve in Vivo Imaging. 2019, ACS Chem. Biol., Yeh HW, Xiong Y, Wu T, Chen M, Ji A, Li X, Ai HW. abstract  article
• Identification of Factors Complicating Bioluminescence Imaging. 2019, Biochemistry, Yeh HW, Wu T, Chen M, Ai HW. abstract  article
• Click beetle luciferase mutant and near infrared naphthyl-luciferins for improved bioluminescence imaging. 2018, Nat Commun, Hall MP, Woodroofe CC, Wood MG, Que I, Van't Root M, Ridwan Y, Shi C, Kirkland TA, Encell LP, Wood KV, Löwik C, Mezzanotte L, Mezzanotte L. abstract  article

Highly pure D-Luciferin potassium salt. This potassium salt is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by luc gene.

Highly pure D-Luciferin potassium salt. This potassium salt is a water soluble form of D-Luciferin. D-Luciferin is a common bioluminescent substrate used for imaging of firefly luciferase expression with peak chemiluminescent emission at 560 nm. The ATP dependant luciferin oxidation is catalyzed by the Luciferase enzyme encoded by luc gene. Properties 99% pure D-Luciferin as measured by HPLC and chiral HPLC